Assembly and processing of subunit II (PsaD) precursor in the isolated photosystem-I complex
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چکیده
منابع مشابه
Multiple functions for the C terminus of the PsaD subunit in the cyanobacterial photosystem I complex.
PsaD subunit of Synechocystis sp PCC 6803 photosystem I (PSI) plays a critical role in the stability of the complex and is part of the docking site for ferredoxin (Fd). In the present study we describe major physiological and biochemical effects resulting from mutations in the accessible C-terminal end of the protein. Four basic residues were mutated: R111, K117, K131, and K135, and a large 36-...
متن کاملThe PsaD Subunit of Photosystem I ' Mutations in the Basic Domain Reduce the Leve 1 of PsaD in the Membranes
Fd. PsaD and PsaE facilitate Fd docking, and PsaE may be The PsaD subunit of photosystem I (PSI) i s a peripheral protein involved in cyclic electron flow "nd PSI (Chimis, 1996). that provides a docking site for ferredoxin and interacts with the PsaD is a crucial component on the reducing side of PSI. PsaB, PsaC, and PsaL subunits of PSI. We used site-directed muThe insertional inactivation of ...
متن کاملThe PsaD subunit of photosystem I. Mutations in the basic domain reduce the level of PsaD in the membranes.
The PsaD subunit of photosystem I (PSI) is a peripheral protein that provides a docking site for ferredoxin and interacts with the PsaB, PsaC, and PsaL subunits of PSI. We used site-directed mutagenesis to determine the function of a basic region in PsaD of the cyanobacterium Synechocystis sp. PCC 6803. We generated five mutant strains in which one or more charged residues were altered. Western...
متن کاملSynthesis and Assembly of the Polypeptides of Photosystem I and II in Isolated Etiochloroplasts of Wheat.
Synthesis and assembly of photosystems (PS) I and II polypeptides in etiochloroplasts isolated from greening wheat (Triticum aestivum L. cv Norin 61) seedlings were studied. The isolated etiochloroplasts synthesized PSI polypeptides of 66 and 15 kilodaltons, PSII polypeptides of 46 and 42 kilodaltons, and atrazine-binding 34 to 32 kilodalton polypeptide. Their assembly processes in the thylakoi...
متن کاملSubunit stoichiometry of the chloroplast photosystem I complex.
A native photosystem I (PS I) complex and a PS I core complex depleted of antenna subunits has been isolated from the uniformly 14C-labeled aquatic higher plant, Lemna. These complexes have been analyzed for their subunit stoichiometry by quantitative sodium dodecyl sulfate-polyacrylamide gel electrophoresis methods. The results for both preparations indicate that one copy of each high molecula...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1992
ISSN: 0014-5793
DOI: 10.1016/0014-5793(92)80273-j